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Elabscience Biotechnology
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Cusabio
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R&D Systems
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Journal: Bioactive Materials
Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration
doi: 10.1016/j.bioactmat.2026.02.059
Figure Lengend Snippet: Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.
Article Snippet: ELISA kits for PGE2 (Cat. No. E-EL-0034), TGF-β (Cat. No. E-EL-0162), VEGF (Cat. No. E-EL-R2603), and
Techniques: Confocal Microscopy, Fluorescence, Staining, Cell Culture, Expressing
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Development of a biomimetic thyroid acellular scaffold as a 3D platform for modeling thyroid cancer aggressiveness and drug resistance
doi: 10.3389/fbioe.2025.1692549
Figure Lengend Snippet: Retention of endogenous growth factors in the Thyroid Acellular Scaffold (TAS). The bar chart shows the concentrations (pg/mL) of key growth factors—Vascular Endothelial Growth Factor (VEGF), Transforming Growth Factor-β (TGF-β), Hepatocyte Growth Factor (HGF), Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), and Platelet-Derived Growth Factor (PDGF)—in native thyroid tissue and the TAS, as quantified by ELISA. Although the decellularization process resulted in a significant reduction in the concentration of all measured growth factors, the TAS successfully retained a substantial amount of these bioactive molecules. Data are presented as mean ± standard deviation. **p < 0.01 compared to native tissue.
Article Snippet: Specific ELISA kits were used to detect the content of vascular endothelial growth factor (VEGF, R&D Systems, RRV00), transforming growth factor-β (TGF-β, R&D Systems, RTB100B),
Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation